Hassan, H., Ebnalwaled, K., Gedawy, H., Mahrous, N. (2024). THE THERAPEUTIC EFFECTS OF MAGNETIC NANOPARTICLES HYPERTHERMIA ON LIVER CANCER : IN VITRO AND IN VIVO STUDIES. Egyptian Journal of Zoology, 81(81), 76-90. doi: 10.21608/ejz.2023.224673.1101
Hemely A. Hassan; Khaled Ebnalwaled; Huda Y. Gedawy; Nadia S. Mahrous. "THE THERAPEUTIC EFFECTS OF MAGNETIC NANOPARTICLES HYPERTHERMIA ON LIVER CANCER : IN VITRO AND IN VIVO STUDIES". Egyptian Journal of Zoology, 81, 81, 2024, 76-90. doi: 10.21608/ejz.2023.224673.1101
Hassan, H., Ebnalwaled, K., Gedawy, H., Mahrous, N. (2024). 'THE THERAPEUTIC EFFECTS OF MAGNETIC NANOPARTICLES HYPERTHERMIA ON LIVER CANCER : IN VITRO AND IN VIVO STUDIES', Egyptian Journal of Zoology, 81(81), pp. 76-90. doi: 10.21608/ejz.2023.224673.1101
Hassan, H., Ebnalwaled, K., Gedawy, H., Mahrous, N. THE THERAPEUTIC EFFECTS OF MAGNETIC NANOPARTICLES HYPERTHERMIA ON LIVER CANCER : IN VITRO AND IN VIVO STUDIES. Egyptian Journal of Zoology, 2024; 81(81): 76-90. doi: 10.21608/ejz.2023.224673.1101
THE THERAPEUTIC EFFECTS OF MAGNETIC NANOPARTICLES HYPERTHERMIA ON LIVER CANCER : IN VITRO AND IN VIVO STUDIES
1Zoology Department, Faculty of Science, South Valley University, Qena, Egypt
2Electronics and Nano Devices Lab, Physics Department, Faculty of Science, South Valley University, Qena, Egypt
3Faculty of Nanotechnology for Postgraduate Studies, Cairo University, Sheikh Zayed Campus, Giza, Egypt
Abstract
Magnetic fluid hyperthermia is a modern cancer treatment that selectively heats tumor tissues to destroy them without harming healthy tissues. The current study aimed to evaluate the cytotoxic effect of Fe3O4/chitosan nanocomposite hyperthermia on liver cancer both in vitro and invivo. The nanocomposite was prepared using the co-precipitation technique; and its magnetic and optical properties were measured along with its Raman spectrum. Cell toxicity for Fe3O4/chitosan nanocomposite was conducted on a liver cancer cell line (HepG2) using 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay, and the cell viability was performed after exposure to Fe3O4/chitosan nanocomposite hyperthermia using trypan blue stain. For the in vivo experiments, 25 male BALB/c albino micewere randomly/equally allotted into five groups: the 1st group was the non-tumor control group, the 2nd group involved the tumor-bearing control mice, the 3rd group involved the tumor-bearing mice received intramuscular injection of Fe3O4/chitosan (90 mg/kg, once/week for two weeks), the 4th and 5th groups involved tumor-bearing mice received intramuscular injection of Fe3O4/chitosan and exposed to an alternating magnetic field with a frequency of "200 kHz" and an output current of "300 A" once or twice/week, respectively, for two weeks. The results showed that nanocomposite was able to induce cytotoxicity (in vitro), as well as enhanced programmed cell death and necrosis of tumor cells, and reduced significantly (P<0.05) the tumor size (in vivo), when exposed to a hyperthermal magnetic field. In conclusion, Fe3O4/chitosan nanocomposite could be a promising therapeutic option for liver cancer through magnetic heating technology.
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