THE POSSIBLE AMELIORATIVE EFFECT OF MESENCHYMAL STEM CELLS AGAINST HEPATO-RENAL TOXICITY IN RATS

Document Type : Original Research Papers

Authors

1 Department of Biochemistry, National Organization for Drug Control and Research, Egypt

2 Department of Physiology, National Organization for Drug Control and Research, Egypt

3 Department of Biochemistry, Faculty of Medicine, Cairo University, Giza, Egypt

Abstract

This study aimed to evaluate the effect of bone marrow-derived mesenchymal stem cells (BMSCs) on the possible repair and prevention of acute hepato-renal injury induced by garamycin (GM). Animals received daily injection of GM for 20 days. On the 10thday, BMSCs were administered. Levels of serum creatinine (Cr), blood urea (Ur) and serum uric acid (UA), as well as ALT and AST activities were determined. The kidney and liver were histopathologically studied using hematoxylin/eosin staining dye. The results showed that the levels of serum Cr, Ur and UA, as well as ALT and AST activities were significantly increased during GM administration. The BMSCs transplantation allevaited the increase in serum Cr and Ur levels, and the incidence of necrosis and apoptosis, as well as increased cell proliferation. The pro-inflammatory cytokines levels decreased, while the anti-inflammatory cytokines levels increased in GM plus BMSCs treated group as compared to the positive control group (GM). Changes in blood minerals levels detected metabolic imbalances that occured in severe kidney dysfunction such as an increase in blood acidity (acidosis, which is caused by a low bicarbonate level), a low potassium level (hypokalemia), a low sodium level (hyponatremia) and a high calcium level. In conclusion, the BMSCs minimized the GM-induced hepato-renal dysfunction. The use of BMSCs can be a potential therapeutic tool for hepato-nephrotoxicity in the near future.

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Egyptian Journal of Zoology
Volume 62, Issue 62
December 2014
Pages 163-178

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