Aziz, A. (2014). BIODISTRIBUTION AND FUNCTIONAL REACTIVITY OF SOME IMMUNE CELLS IN PEYER’S PATCHES, CECAL PATCHES AND SPLEEN OF ALBINO MICE AFTER TREATMENT WITH THE NANOPARTICLES. Egyptian Journal of Zoology, 62(62), 27-48. doi: 10.12816/0009336
Aziz Awaad Aziz. "BIODISTRIBUTION AND FUNCTIONAL REACTIVITY OF SOME IMMUNE CELLS IN PEYER’S PATCHES, CECAL PATCHES AND SPLEEN OF ALBINO MICE AFTER TREATMENT WITH THE NANOPARTICLES". Egyptian Journal of Zoology, 62, 62, 2014, 27-48. doi: 10.12816/0009336
Aziz, A. (2014). 'BIODISTRIBUTION AND FUNCTIONAL REACTIVITY OF SOME IMMUNE CELLS IN PEYER’S PATCHES, CECAL PATCHES AND SPLEEN OF ALBINO MICE AFTER TREATMENT WITH THE NANOPARTICLES', Egyptian Journal of Zoology, 62(62), pp. 27-48. doi: 10.12816/0009336
Aziz, A. BIODISTRIBUTION AND FUNCTIONAL REACTIVITY OF SOME IMMUNE CELLS IN PEYER’S PATCHES, CECAL PATCHES AND SPLEEN OF ALBINO MICE AFTER TREATMENT WITH THE NANOPARTICLES. Egyptian Journal of Zoology, 2014; 62(62): 27-48. doi: 10.12816/0009336
BIODISTRIBUTION AND FUNCTIONAL REACTIVITY OF SOME IMMUNE CELLS IN PEYER’S PATCHES, CECAL PATCHES AND SPLEEN OF ALBINO MICE AFTER TREATMENT WITH THE NANOPARTICLES
Department of Zoology, Faculty of Science, Sohag University, Sohag, Egypt
Abstract
Gut-associated lymphoid tissues (GALT) including Peyer's patches (PPs) and cecal patches (CPs) have a vital role in the induction of immune response against pathogens. Spleen is considered as non-GALT; it has a variety of immune cells that support the immune response. In this study, the expression of alpha L-fucose residues and biodistribution of Lectin Ulex Europaeus Agglutinin I (UEA I+) cells, IgA+ cells, 33D1+ dendritic cells (DCs) and CD11b+ macrophage subsets in the GALT (PPs and CPs) and in non-GALT (spleen) were investigated using UEAI lectin histochemistry and immunohistochemistry. The immune response induction of alpha L-fucose residue, UEAI+ cells, IgA+ cells, 33D1+ DCs and CD11+ macrophages subsets also will be investigated after treatment with amino-conjugated fluorescent silica nanoparticles (FSNPs). After treatment with FSNPs an induction of alpha L-fucose residue was observed in the PPs M cells in all cytoplasm, rather than the apical cytoplasm as shown in the control group. Also, the number of UEAI+ cells and IgA+ cells in PPs sub-epithelial dome (SED) increased 4 times and 2 times higher, respectively, as compared with that of the control group. The CD11b+ macrophages, but not 33D1+ DCs subsets located in PPs SED, had the main role to uptake FSNPs. In the case of spleen, the number of 33D1+ DCs and CD11b+ macrophages subsets was 30 times and 25 times higher, respectively, as compared with that of control group. These data indicated that alpha L-fucose residue and IgA+ cells, 33D1+ DCs and CD11b+ macrophages subsets in PPs as GALT and spleen as non-GALT had important role, but in different ratios, in the immune system induction against FSNPs.
View on SCiNiTO
Top