PATHOGENICITY OF PHOTORHABDUS LUMINESCENS, A SYMBIOTIC BACTERIUM OF AN ENTOMOPATHOGENIC NEMATODE, AGAINST GALLERIA MELLONELLA L. AND SPODOPTERA LITTORALIS (BOISD.)

Document Type : Original Research Papers

Authors

1 Plant Protection Research Institute, Biological Control Department, Agriculture Research Center, Giza, Egypt

2 Pests and Plant Protection Department, National Research Centre, Giza, Egypt

Abstract

The Photorhabdus luminescens, a symbiotic bacterium of an entomopathogenic nematode (EPNs), Heterorhabditis bacteriophora BA1 was evaluated against greater wax moth, Galleria mellonella L. and the cotton leaf worm, Spodoptera littoralis (Boise.). P. luminescens is a gram-negative bacterium, belonging to the family Enterobacteriaceae. In this study, a fresh primary phase (Phase I) of P. luminescens was isolated from a pool of 500 infective juveniles (IJs) of H. bacteriophora and its efficiency was compared to 2-years old bacterium against 3rd larval instars of G. mellonella and S. littoralis under laboratory conditions. The virulence of P. luminescens to S. littoralis larvae was higher than that to G. mellonella larvae and mortality percentage increased as the concentration of (EPB) increased.The fresh bacteriumwasmore effective than 2-years old one. LC50 were found to be 1.31x108 and 1.512x108 bacterial cells/ml for S. littoralis and 1.78x108 and 1.95x108 bacterial cells/ml for G. mellonella treated with fresh P. luminescens and 2-years old one, respectively. 

Keywords

Main Subjects

Files

Share

How to cite

Statistics