bdel-Latif, M., Abdel-Aziz, A., Taha, R., El-Mallah, A., Sami, S. (2016). FILARIAL EXCRETORY-SECRETORY ANTIGENS INDUCE MURINE PRODUCTION OF IFN-GAMMA AND IL-15. Egyptian Journal of Zoology, 65(65), 65-88. doi: 10.12816/0027818
Mahmoud bdel-Latif; Ayman M. Abdel-Aziz; Ragaa Taha; Al-Mahy El-Mallah; Sara Sami. "FILARIAL EXCRETORY-SECRETORY ANTIGENS INDUCE MURINE PRODUCTION OF IFN-GAMMA AND IL-15". Egyptian Journal of Zoology, 65, 65, 2016, 65-88. doi: 10.12816/0027818
bdel-Latif, M., Abdel-Aziz, A., Taha, R., El-Mallah, A., Sami, S. (2016). 'FILARIAL EXCRETORY-SECRETORY ANTIGENS INDUCE MURINE PRODUCTION OF IFN-GAMMA AND IL-15', Egyptian Journal of Zoology, 65(65), pp. 65-88. doi: 10.12816/0027818
bdel-Latif, M., Abdel-Aziz, A., Taha, R., El-Mallah, A., Sami, S. FILARIAL EXCRETORY-SECRETORY ANTIGENS INDUCE MURINE PRODUCTION OF IFN-GAMMA AND IL-15. Egyptian Journal of Zoology, 2016; 65(65): 65-88. doi: 10.12816/0027818
FILARIAL EXCRETORY-SECRETORY ANTIGENS INDUCE MURINE PRODUCTION OF IFN-GAMMA AND IL-15
1Department of Zoology, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt
2Department of Zoology, Faculty of Science, Fayoum University, Fayoum, Egypt
Abstract
Filarial nematodes are responsible for an annual global health burden of ∼6.3 million people. We studied the effect of excretory-secretory products (ES) of the female worm Setariaequina and the drug diethylcarbamazine citrate (DEC) and their combination on the production of interferon-gamma (IFN-γ) and interleukin-10 (IL-10). Seventy male Swiss mice, (22 ± 2g) of about 4-6 weeks old were used and divided into seven groups each one consisted of 10 male mice. The first group (control) received 1 ml tyrode's solution, the second group received 0.25 ml ES, the third group received 1 ml ES, the fourth group received 25 mg/kg DEC in 0.25 ml tyrode's solution, the fifth group received 100 mg/kg DEC in 1 ml tyrode's, the sixth group received 25 mg/kg DEC in 0.25 ml ES and the seventh group received 100 mg/kg DEC in 1 ml ES. Exvivo assay results showed that both 0.25 ml and 1 ml of ES could induce the highest level of IFN-γ release compared to the control group (P<0.001). In contrast, the same mice groups showed the lowest decrease in IL-10 levels (P<0.001). For invivo tests, serum levels of IFN-γ were the highest in all groups, while IL-10 levels were the lowest in ES mice groups (P<0.001). Immunoblotting has shown a higher expression of Interleukin-15 (IL-15) in ES mice groups with observably higher expression in 1 ml ES than 0.25 ml ES (P<0.001). IL-15 protein was localized using immunohistochemistry, where the staining pattern in liver suggested that tissue resident Kupffer cells were the main producers and they were present in abundance in mice receiving high dose of ES.
View on SCiNiTO
Top