SUBLETHAL EFFECTS OF THE ESSENTIAL OILS OF GARLIC (ALLIUM SATIVUM) AND LEMON (CITRUS LIMON) ON NUTRITIONAL INDICES AND MIDGUT HISTOLOGICAL AND ULTRASTRUCTURAL CHANGES IN THE EGYPTIAN COTTON LEAFWORM (SPODOPTERA LITTORALIS)

Ali, Ali Mohamed; Shaurub, El-Sayed H.; Mohamed, D. S.; El-Sayed, Asmaa Metwaly

doi:10.12816/0043201

Home Articles List Article Information

|
|
|
How to cite
RIS EndNote BibTeX APA MLA Harvard Vancouver
|
Share

Articles in Press

Current Issue

Journal Archive

Volume 81 (2024)

Volume 80 (2023)

Volume 79 (2023)

Volume 78 (2022)

Volume 77 (2022)

Volume 76 (2021)

Volume 75 (2021)

Volume 74 (2020)

Volume 73 (2020)

Volume 72 (2019)

Volume 71 (2019)

Volume 70 (2018)

Volume 69 (2018)

Volume 68 (2017)

Issue 68

Volume 67 (2017)

Volume 66 (2016)

Volume 65 (2016)

Volume 64 (2015)

Volume 63 (2015)

Volume 62 (2014)

Volume 61 (2014)

Volume 60 (2013)

Volume 59 (2013)

	SUBLETHAL EFFECTS OF THE ESSENTIAL OILS OF GARLIC (ALLIUM SATIVUM) AND LEMON (CITRUS LIMON) ON NUTRITIONAL INDICES AND MIDGUT HISTOLOGICAL AND ULTRASTRUCTURAL CHANGES IN THE EGYPTIAN COTTON LEAFWORM (SPODOPTERA LITTORALIS)
Article 18, Volume 68, Issue 68, December 2017, Page 339-361
Document Type: Original Research Papers
DOI: 10.12816/0043201
View on SCiNiTO
Authors
Ali Mohamed Ali¹; El-Sayed H. Shaurub ²; D. S. Mohamed¹; Asmaa Metwaly El-Sayed¹
¹Department of Zoology, Faculty of Science, Assiut University, Assiut, Egypt
²Department of Entomology, Faculty of Science, Cairo University, Giza, Egypt
Abstract
The present study was carried out to evaluate the nutritional indices, histological (light microscopy) and ultrastructural (transmission electron microscopy) changes in the midgut of the 4^th larval instar of Spodoptera littoralis treated with the LC₃₀ of the essential oil of garlic (Allium sativum) (16.60×10⁴ mg/l) and lemon (Citrus limon) (20.09×10⁴ mg/l) based on leaf–dip method compared to untreated larvae (control). The results of the nutritional metabolism revealed a significant reduction in food consumption index (CI), relative growth rate (RGR), and approximate digestibility (AD) of treated larvae. On the contrary, the efficiency of conversion of ingested food to body mass (ECI) and the efficiency of conversion of digested food to body mass (ECD) increased significantly in treated larvae. The most prominent symptoms revealed by light microscopy on the midgut of the treated larvae were vacuolization of the epithelial layer, clumping of the nuclear chromatin material and degeneration of the peritrophic membrane and microvilli. On the other hand, transmission electron microscopy showed vacuolization and degeneration of the columnar cells, clumping of the nuclear chromatin, breaking of microvilli, loss of the cytoplasmic projections of the goblet cells, appearance of residual bodies, and disintegration of mitochondria. In particular, treatment with lemon oil resulted in the appearance of numerous lysosomes and electron-dense mitochondrial matrices. In conclusion, the reduction of the RGR would result in undersized females in the next generations; in turn this would lead to a reduction of the reproductive potential. Also, the present investigation indicates that the microvilli and/or mitochondria are the probable target site(s) of action of garlic and lemon essential oils.
Keywords
<i>Spodoptera littoralis</i>; Essential oils; Nutritional Indices; Midgut; histology; Ultrastructure
Main Subjects
Animal Histology; Animal Nutrition and Biochemistry; Arthropods


Statistics Article View: 249